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Pharmaceutical Chemistry and Technology

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LABORATORIO DI BIOTECNOLOGIE FARMACEUTICHE - LABORATORIO DI PREPARAZIONE ESTRATTIVA E SINTETICA DEI FARMACI A - L

Academic Year 2021/2022 - 5° Year
Teaching Staff Credit Value: 12
Scientific field: CHIM/08 - Pharmaceutical chemistry
Taught classes: 56 hours
Laboratories: 60 hours
Term / Semester: 2°

Learning Objectives

  • LABORATORIO DI BIOTECNOLOGIE FARMACEUTICHE

    The course aims to provide the basic knowledge of biotechnology methodologies for the design and the development of biotechnological drugs. The student will acquire the knowledge necessary to understand the principles for the expression of recombinant proteins in different expression systems (i.e., bacterial, yeast, insect, and mammalian), as well as the production, formulation, and characterization of biopharmaceuticals. At the end of the course, the student should develop fundamental laboratory skills and will be able to perform basic approaches for the manipulation of gene expression.

  • LABORATORIO DI PREPARAZIONE ESTRATTIVA E SINTETICA DEI FARMACI

    Provide the concepts and general strategies of retrosynthetic analysis for the resolution of complex synthetic problems through rational analysis; provide a basic experience in purification methods, identification and characterization of reaction and extraction products.

    Acquired skills: theoretical and practical knowledge concerning the synthesis of organic compounds endowed with pharmacological activity. Theoretical and practical knowledge on purification methods and identification of the synthesized compounds.


Course Structure

  • LABORATORIO DI BIOTECNOLOGIE FARMACEUTICHE

    The course includes 28 hours of lectures and 30 hours of laboratory classes.

    Class attendance is mandatory. The maximum limit of absences allowed is 30% of the total hours of lessons.

    Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.

    The final exam is oral. Learning assessment may also be carried out on line, should the conditions require it.

  • LABORATORIO DI PREPARAZIONE ESTRATTIVA E SINTETICA DEI FARMACI
    • Frontal lessons 28 hours
    • Laboratory practice 30 hours

    Should teaching be carried out in mixed mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus.

    Class attendance is mandatory. The maximum limit of absences allowed is 30% of the total hours of lessons.

    The final exam is written. Learning assessment may also be carried out on line, should the conditions require it.

    Information for students with disabilities and / or SLD:
    To guarantee equal opportunities and in compliance with the laws in force, interested students can ask for a personal interview in order to plan any compensatory and / or dispensatory measures, based on the teaching objectives and specifications needs. It is also possible to contact the CInAP contact person (Center for Active and Participatory Integration - Services for Disabilities and / or SLD) of the Department, prof.ssa Teresa Musumeci.


Detailed Course Content

  • LABORATORIO DI BIOTECNOLOGIE FARMACEUTICHE
    • Biotechnological and biomolecular methods for drug design and production. Gene manipulation and recombinant DNA technology.
    • Manipulation of genic expression and production of recombinant protein in prokaryotic and eukaryotic cells.
    • Downstream processing of protein products.
    • Therapeutic proteins: definition and classification. Hematopoietic growth factors. Interferons and interleukins. Insulin. GLP-1 analogues. Vaccines. Monoclonal antibodies.
    • Oligonucleotides (ON): The medicinal chemistry of ON, pharmacokinetics and toxicology. Antisense technology, aptamers, decoy, anti-genes, ribozymes, RNA-interference, micro-RNA.
    • Principles of structural bioinformatics.
  • LABORATORIO DI PREPARAZIONE ESTRATTIVA E SINTETICA DEI FARMACI
    • Drugs of extractive, synthetic and semi-synthetic origin.
    • Design of a synthetic method: means and development of bibliographic research.
    • Retrosynthetic analysis.
    • Detailed analysis of the syntheses and reaction mechanisms involved in the preparation of compounds for therapeutic use and in the synthesis of molecules of biological origin: strategies and methods.
    • Common equipments, instrumentations and laboratory methods.
    • Translation of a synthetic plan into practical applications.
    • Synthesis of some simple organic compounds.
    • Synthesis of organic compounds with potential or known pharmacological activity.
    • Application of different chromatographic methods and analytical and spectroscopic techniques for purification and identification of synthesized compounds

Textbook Information

  • LABORATORIO DI BIOTECNOLOGIE FARMACEUTICHE
    1. Bibliography and notes provided during the course available online at: http://studium.unict.it/dokeos/2021/
    2. D. J. A. Crommelin & R. D. Sindelar, BIOTECNOLOGIE FARMACEUTICHE, Zanichelli.
    3. B. R. Glick, J. J. Pasternak, BIOTECNOLOGIA MOLECOLARE, Zanichelli.
    4. M. L. Calabrò, Compendio di BIOTECNOLOGIE FARMACEUTICHE, EdiSES.
    5. A. Maggi, BIOTECNOLOGIE FARMACOLOGICHE, MASSON.
    6. E. Vegeto, A. Maggi, P. Minghetti, FARMACI BIOTECNOLOGICI: aspetti farmacologici e clinici, Casa Editrice Ambrosiana.
    7. T. A. Brown, Biotecnologie molecolari: Principi e tecniche, Seconda edizione italiana, Zanichelli.
    8. G. A. Petsko, D. Ringe, Struttura e funzione delle proteine, Zanichelli.
    9. D. J. A. Crommelin, R. D. Sindelar, B. Meibohm. Pharmaceutical Biotechnology: Fundamentals and Applications, Fifth Edition. Springer.
    10. G. Walsh. Pharmaceutical Biotechnology: Concepts and Applications, Wiley.
    11. T. Brown & Tom Brown (Jnr). Nucleic Acids Book, Atdbio. https://www.atdbio.com/nucleic-acids-book.
    12. J. Sambrook, E. F. Fritsch, T. Maniatis, Molecular cloning: A laboratory manual, Second edition, Cold Spring Harbor Laboratory Press
  • LABORATORIO DI PREPARAZIONE ESTRATTIVA E SINTETICA DEI FARMACI
    • Stuart Warren, Paul Wyatt - Organic synthesis -The Disconnection Approach, 1st and 2nd Edition - J. Wiley & Sons (1982/2009).
    • Stuart Warren, Paul Wyatt - Workbook for Organic Synthesis The Disconnection Approach, 1st and 2nd Edition - J. Wiley & Sons (1982/2009).
    • Experimental Organic Chemistry: A Miniscale & Microscale Approach, 6th Edition. John C. Gilbert, Stephen F. Martin. ISBN: 9781305080461
    • A Small Scale Approach to Organic Laboratory Techniques, 4th Edition. Donald L. Pavia, George S. Kriz, Gary M. Lampman, Randall G. Engel. ISBN: 9781305253926.