ANALISI BIOCHIMICO CLINICO-MOLECOLARI

Dublin descriptors

D1. Knowledge and understanding: of the specific language of the discipline; the main metabolic pathways involved in Laboratory Medicine; identification and practical use of Biomarkers in acute and chronic organ diseases.

D2. Ability to apply knowledge and understanding: knowing how to entertain debates and interactive discussions in the classroom; be able to identify research topics through bibliographic research.

D3. Judgment skills: knowing how to develop and manage a critical reflection on the alteration of some metabolic pathways.

D4. Communication skills: knowing how to better manage communication using a specific technical language of the discipline

D5. Learning skills: acquire adequate skills for the analysis and critical evaluation of biomarkers useful for diagnosis and / or prognosis, with a view to patient-centric approach and medicine 4 or 7P. Demonstrate the ability to search for useful information in clinical and research problem solving; application skills of the acquired knowledge
 

Interactive Lectures with use of ppt slides containing movie clips, links to Web sites and lecture.

Should teaching be carried out in hybrid mode or remotely, it may be necessary to introduce changes with respect to previous statements, in line with the programme planned and outlined in the syllabus

The final exam will be oral

Learning assesment may also be carried out electronically, should the conditions require it.

"Information for students with disabilities and / or LSD"

To guarantee equal opportunities and in compliance with the laws in force, students concerned can ask for a personal meeting in order to plan any compensatory tools and / or dispensatory measures, based on the didactic objectives and individual needs. For further information, it is also possible to contact the referent professor for  CInAP (Center for Active and Participatory Integration - Services for Disabilities and / or LSD) of our Department, Prof. Tprof.ssa S. Chiechio. "

Knowledge of cell biology, structure and function of proteins, lipids, nucleic acids, replication, transcription, translation. Passing of Biology and Molecular Biology exams.

Mandatory attendance according to the rules of the didactic regulations of the CdS in CTF

- Analysis laboratory: organization, diagnostic tests and laboratory tests.
- The biological sample: characteristics; collection, treatment and storage. Conventional biological matrices (blood, urine and faeces) and unconventional (reduced invasiveness: saliva, sweat, hair, nails).
- The analytical cycle: quality control, pre-analytical, analytical, post-analytical and reporting variability. Specificity and Diagnostic Sensitivity (ROC Curve). Predictive value. Cut-off, Reference Range and Decision Levels. Basics of statistics.
- POCT (Point-Of-Care Testing): principles and clinical applications.
- Main analytical methods: theoretical principles, state of development, expected effects in clinical diagnostics and applications in research.
- Biomarkers: What they are and their role in clinical diagnostics. Exogenous and endogenous biochemical markers. Measurement of markers of function and injury of the main organs and tissues (Heart, Pancreas, Liver, Kidney, Muscle, Bone). Markers of pathological processes (acute and chronic inflammation), tumor, neurodegenerative, intestinal pathologies and relations with the Microbiota.
- Liquid biopsy: circulating cells, circulating nucleic acids, miRNAs and exosomes.
- Methods for monitoring: drugs, toxic agents, substances of abuse and doping in athletes.
- Vitamins and Minerals: dosage in research protocols and in the clinical setting. Mineralogram and validity of its use.
- Notes on Omics Techniques: lipidomics, exome (whole and clinical), exposome, proteome, microbiome, metabolomics, salivomics.

- Ciaccio-Lippi, Biochimica clinica e medicina di laboratorio. EDISES Eds 2017
- Sacchetti, Cavalcanti, Fortunato, pastore, Rossano, Salvatore, Scopacasa. Medicina di Laboratorio & Diagnostica Genetica. Ed. Sorbona
-  Balestrieri C- Diagnostica molecolare nella Medicina di Laboratorio - Vol IX Edizioni Piccin
- LaPosata: Medicina di Laboratorio: diagnosi di malattia nel laboratorio clinico. Ed Piccin

The final exam will be oral and based on lectures, slides and textbooks

Verification of learning can also be an online assessment, if conditions require it.

Sample-related variables;

Transaminase determination

Glycated hemoglobin and clinical utility

Genomic approaches in the study of genetic disorders

Monitoring of substances of abuse